Presentation I

George Emanuel, Vizgen 
"Cell Atlases Need a Third Dimension: Mapping Cellular Neighborhoods in 3D"

Learn how Volumetric Tissue Mapping from Vizgen enables richer cell atlases by mapping transcripts across 50–100 µm of tissue. Discover cellular neighborhoods, interactions, and spatial organization that can be missed in 2D sections. See how single-molecule sensitivity and subcellular resolution are maintained throughout thick tissue to reveal molecular patterns, gradients, and rare cell populations.

Presentation II

Emily Martersteck, BioTechne Spatial 
"Transforming Tissue into Insight with Same-Section Spatial Multiomics" 

This presentation highlights Bio-Techne Spatial solutions for detecting RNA and protein markers in the same tissue section at subcellular resolution. By combining RNAscope™ single-molecule RNA detection with highly multiplexed protein profiling on the fully automated COMET™ platform, researchers can gain deeper spatial insights into tissue biology.

Presentation III

Alec Barrett, Takara Bio USA, Inc.
"Cross-platform comparison of spatial transcriptomics technologies in an FFPE lung squamous cell carcinoma"

Spatial transcriptomics provides a new perspective on how cells organize and communicate in situ, but platforms differ widely in resolution, sensitivity, and specificity. Using a single real-world FFPE lung squamous cell carcinoma specimen, this benchmarking study compared Takara Bio’s Trekker FX technology with Visium HD and Xenium 5K to evaluate differences in spatial transcriptomics performance. Trekker FX, which uses a novel spatial tag donation-based approach to enable true single-cell-resolution spatial profiling, showed higher whole-transcriptome sensitivity, more accurate detection of key cell markers, and better identification of critical immune and endothelial cell populations and ligand-receptor interactions, providing deeper insights into cancer cell communication networks.